Immune response against DNA- and mRNA vaccines encoding artificial influenza virus immunogens

Constant antigenic drift of circulating influenza viruses leads to inefficiency seasonal vaccines, thus requiring annual re-design these vaccines. Therefore, the development a universal vaccine is particular relevance. A promising line research in this area design immunogens consisting conserved protein fragments from different viral strains. The aim work was assess immunogenicity DNA vaccines and mRNA encoding artificial antigens hemagglutinin stem M2 protein. We have obtained constructs AgH1, AgH3, AgM2, which contained stalk two subtypes H1N1 H3N2, These were used as templates for synthesis To constructs, BALB/c mice immunized with by i/m administration. Assessment humoral immune response carried out ELISA, using A/Aichi/2/68(H3N2), A/California/07/2009 ULTRIX containing purified H3N2 viruses. T cell assessed methods: intracellular cytokine staining (ICS) ELISpot. ICS performed determine CD8+ CD4+T-lymphocytes producing IFN. ELISpot mouse IFN kit (BD). peptide mixture included composition target antigens, stimulation. results showed that designed induce virus-specific cellular responses mice. Intramuscular administration naked induced weak response, suggesting need further improve delivery approaches.